Dr. Douglas Craig
Title: Professor
Phone: 204.786.9731
Office: 2RC016 - Lab: 3RC029
Building: Richardson College for the Environment and Science Complex
Email: d.craig@uwinnipeg.ca
Degrees:
PDF Manitoba 1993-1994, Alberta 1994-1997
Ph.D. Dalhousie 1993
B.Sc. Mount Allison 1988
Biography:
Dr. Doug Craig has been lecturing biochemistry courses at Â鶹´«Ã½ for more than 20 years. He has also received over 25 Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery, Research and Equipment grants, totaling over $1,000,000.
Courses:
CHEM 1111 Introduction to the Chemical Properties of Matter
CHEM 1112 Basic Principles in Chemical Reactivity
CHEM-1111L Introduction to Chemical Properies - Lab Coordinator
CHEM-1112L Basic Chemical Reactivity - Lab Coordinator
CHEM-2202L Organic Chemistry I - Lab Coordinator
CHEM 2302 Quantitative Chemical Analysis
CHEM 3502 Intermediate Biochemistry I
CHEM-3502L Intermediate Biochemistry - Lab Coordinaor
CHEM 3503 Intermediate Biochemistry II
CHEM 3504 Plant Biochemistry
CHEM 3701 Directed Studies in Chemistry
CHEM 4502 Molecular Enzymology
CHEM 4506 Methods in Biochemistry
CHEM-4506 Methods in Biochemistry - Lab
CHEM 4701 Research Projects in Chemistry
Research Interests:
SINGLE MOLECULE ENZYMOLOGY - Chemical studies typically involve measurements of large ensembles of molecules with data representing averages. With respect to enzymes, we now know such average values represent a simplification. Single enzyme molecule assay utilizing ultrasensivitive capillary electrophoresis laser-induced fluorescence instrumentation have demonstrated that individual enzyme molecules are not identical. Individual molecules differ with respect to rate and activation energy of catalysis. Dr. Craig's research interests are in the study of the basis and function of the heterogeneity of enzyme molecules and its role within the cell. A second interest is the development of ultrasensitive methods for the analysis of biological molecules, particularly enzymes and proteins.
Website
Recent Research Funding (Since 2009):
NSERC Discovery grant (5th installment), 2021
NSERC Discovery grant (deferred), 2020
NSERC RTI (equipment) grant (as co-applicant), 2019
NSERC Discovery grant (4th installment), 2019
NSERC Discovery grant (3rd installment), 2018
NSERC Discovery grant (2nd installment), 2017
NSERC Discovery grant (1st installment), 2016
NSERC Discovery grant (7th installment), 2015
NSERC Discovery grant (6th installment), 2014
NSERC Discovery grant (5th installment), 2013
NSERC Discovery grant (4th installment), 2012
NSERC Discovery grant (3rd installment), 2011
NSERC Discovery grant (2nd installment), 2010
University of Winnipeg travel grant, 2010
NSERC Discovery grant (1st installment), 2009
University of Winnipeg travel grant, 2009
Recent Conferences (Since 2014):
73. “Citrate Analysis Using Capillary Electrophoresis and Complexation with Eu3+- Tetracycline” Jennifer R Lischynski, Isabela CC Cardoso & Douglas B Craig. Canadian Society for Chemistry Conference, Quebec, QC, June 2019.
72. “Determination of the Inhibitor Dissociation Constant of an Individual Unmodified Enzyme Molecule in Free Solution” Douglas B Craig, Jeremie J Crawford & Joshua W Hollett, Manitoba Chemistry Symposium, Winnipeg, MB, May 2018.
71. “Measurement of single enzyme molecule inhibitor dissociation constant using capillary electrophoresis separations” DB Craig, JJ Crawford & JW Hollett, ACROSS (Australian Center for Research on Separation Science) International Symposium on Advances in Separation Science, Hobart, Australia, Nov 2016.
70. “Analysis of Complexes of Metabolites with Europium Tetracycline Using Capillary Electrophoresis Coupled with Laser-Induced Luminescence Detection” Z Hiebert & DB Craig, Canadian Society for Chemistry, June 2016, Halifax, NS.
69. “Electrophoretic Mobility, Catalytic Rate, and Activation Energy of Catalysis of Single Molecules of the Enzyme b-Glucuronidase from Escherichia coli” SD King, G Reinfelds, ARP Henderson, TEH Wood & DB Craig, Canadian Society for Chemistry, June 2016, Halifax, NS 68. "12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis" A Henderson, G Renfelds & DB Craig , Canadian Society for Chemistry conference, June 2015, Ottawa, ON.
68. 12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis" A Henderson, G Renfelds & DB Craig , Canadian Society for Chemistry conference, June 2015, Ottawa, ON
67. "Effect of short periods of heating on the catalytic rate and electrophoretic mobility of individual molecules of beta-galactosidase" J Crawford & DB Craig, Canadian society for chemistry conference, June 2015, Ottawa, ON.
66. "The separation of historical dyes using capillary electrophoresis" D Goltz, S Ahmadi, J Crawford, B Ferris & D Craig, Canadian Society for Chemistry conference, June 2015, Ottawa, ON.
65. "12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis" A Henderson, G Renfelds & DB Craig, Association of official analytical chemists meeting, June 2015, Winnipeg, MB.
64. "A look at individual molecules: Beta-galactosidase by capillary electrophoresis coupled with laser-induced fluorescence" JJ Crawford & DB Craig , Association of official analytical chemists meeting, June 2015, Winnipeg, MB.
63. "Measurement of the activity of individual subunits of a single enzyme molecule and the conversion of a molecule between different active conformations" DB Craig, JJ Crawford, C Ong-Justiniano & E Nichols, International conference on analytical sciences and spectroscopy, May 2015, Halifax, NS.
62. "12-Channel Peltier array temperature control unit for capillary electrophoresis" DB Craig, G Renfields & A Henderson, Canadian Society for Chemistry conference, June 2014, Vancouver, BC.
61. "Catalytic and electrophoretic heterogeneity of streptavidin-beta-galactosidase" A Henderson & DB Craig, Canadian Society for Chemistry conference, June 2014, Vancouver, BC.
60. "Post-column protein labelling using a caged cyanide for laser-induced fluorescence capillary electrophoresis" B Vuong, DB Craig & T Wood, Association of Official Analytical Chemists meeting, June 2014, Winnipeg, MB.
Publications:
Recent Publications (Since 2014)
62. JR Lischynski, DM Goltz & DB Craig (2019) Measurement of Phosphate in Small Samples Using Capillary Electrophoresis with Laser-Induced Luminescence Detection. J. Liq. Chromatogr. R.T. 41, 1-6.
61. DB Craig, JR Lischynski, ICC Cardoso (2018) Citrate Analysis Using Capillary Electrophoresis and Complexation with Eu3+-Tetracycline. BioMetals 31, 1043- 1049.
60. DB Craig, SD King, G Reinfelds, ARP Henderson & TEH Wood (2017) Electrophoretic Mobility, Catalytic Rate, and Activation Energy of Catalysis of Single Molecules of the Enzyme b-Glucuronidase from Escherichia coli. International Journal of Biological Macromolecules 96, 669-674.
59. DB Craig & Z Hiebert (2017) Analysis of Complexes of Metabolites with Europium Tetracycline Using Capillary Electrophoresis Coupled with Laser-Induced Luminescence Detection. BioMetals 30, 449-458.
58. JJ Crawford, JW Hollett & DB Craig (2016) Determination of the Inhibitor Dissociation Constant of an Individual Unmodified Enzyme Molecule in Free Solution. Electrophoresis 37, 2217-2225.
57. D Goltz, S Ahmadi, J Crawford & D Craig (2016) Photochemical properties of flavonol dyes: Effects on their separation using capillary electrophoresis with laser induced fluorescence detection. Journal of Liquid Chromatography & Related Technologies 39, 786-774.
56. JJ Crawford, F Itzkow, J MacLean & DB Craig (2015) Conformational Change in Individual Enzyme Molecules. Biochemistry and Cell
55. Craig, DB, Hanlon-Dearman, F, Beaudry, S, Shek, K & King, SD (2015) Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal alkaline phosphatase. Protein Expression & Purification 114, 95-98.
54. Craig, DB, Reinfelds, G & Henderson, A (2014) 12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis. Electrophoresis 35, 2408-2411.
53. Ahmadi, S, Absalan, G, Craig, DB & Goltz, DM (2014) Photochemical properties of purpurin and its implications for capillary electrophoresis with laser induced fluorescence. Dyes and Pigments 105, 57-62.